Protein Purification Reagents and Kits
Sigma Aldrich Fine Chemicals Biosciences L-ARGINASE FROM BOVINE LIV25KU
L-Arginase from bovine liver, 25KU, 9000-96-8
CUBE BIOTECH PureCube Ni-NTA Agarose; 500 ml
500 ml Ni-NTA MagBeads for His-tag-purification, 25% slurry
CUBE BIOTECH PureCube Co-NTA MagBeads; 1 ml
1ml of Co-NTA MagBead slurry (25%) for Protein Purification of His-tagged proteins
CUBE BIOTECH PureCube Epoxy Activated Agarose; 50 ml
50 ml of Epoxy Activated Agaroses for the coupling of biomolecules with an exposed NH2 or SH-group
CUBE BIOTECH PureCube 100 Ni-INDIGO Agarose; 500 ml
500 ml 100 Ni-INDIGO Agarose for His-tag-purification, 50% slurry
Hampton Research Loctite Easy-Squeeze Super Glue Gel
HR4-316/Loctite Easy-Squeeze Super Glue Gel - 4 gram tube. Please note items from this supplier are non-returnable.
Hampton Research HR8-069/AlumaSeal II Sealing Film - 100 pack
HR8-069/AlumaSeal II Sealing Film - 100 pack. Please note items from this supplier are non-returnable.
Hampton Research HR3-215/22 mm x 0.22 mm Siliconized Square Cover Slides - 1.0 ounce pack (approximately 100 slides)
HR3-215/22 mm x 0.22 mm Siliconized Square Cover Slides - 1.0 ounce pack (approximately 100 slides). Please note items from this supplier are non-returnable.
STEMCELL Technologies EasySep™ Mouse CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-CD4+ T cells and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.This product replaces the EasySep™ Mouse CD4+ T Cell Enrichment Kit (Catalog #19752) for even faster cell isolations.
STEMCELL Technologies EasySep™ Mouse Pan-B Cell Isolation Kit
10-Minute cell isolation kit using immunomagnetic negative selection. The EasySep™ Mouse Pan-B Cell Isolation Kit is designed to isolate all B cells, including conventional B-2 B cells, B-1 B cells and plasma cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non pan-B cells (CD4, CD8, CD11c, CD49b, CD90.2, Ly-6C/G (Gr-1), TER119) and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns.This kit is compatible for use with cells from disease models where the malignant cells (B-CLL) express CD43 or CD11b. For isolation of conventional B cells only, we recommend using the EasySep™ Mouse B Cell Isolation Kit (Catalog #19854).Click here to learn about our next-generation EasySep™ mouse cell isolation kits, featuring RapidSphere™ technology.
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC3895568 MISSION PLKO.1-PURO TURBOGFP
System Biosciences LLC PEG-IT VIRUS PRECIPITATION PEG-it Virus Precipitation Solution = 100mL
Easily concentrate lentivirus particles for ultra-high titers. Used in over three-hundred citations, PEG-it™ Virus Concentration Reagent enables easy concentration of pseudoviral particles for achieving ultra-high titers. It’s great for concentrating pseudoviral particles even from large volumes of medium by removing the need for ultracentrifugation. Simply add PEG-it to the collected medium, incubate overnight at 4°C, and spin at 1500g for 30 minutes.In addition, PEG-it acts as a cryopreservative for concentrated virus. Lentivirus concentrated with PEG-it lasts longer in the freezer and survives multiple freeze-thaw cycles with minimal loss of titer.
STEMCELL Technologies EasySep™ Mouse B Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse B Cell Isolation Kit is designed to isolate B cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-B cells and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using and EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For isolation of B cells expressing CD11b or CD43, we recommend using the EasySep™ Mouse Pan-B Cell Isolation kit (Catalog #19844 (https://www.stemcell.com/products/easysep-mouse-pan-b-cell-isolation-kit.html)).This product replaces the EasySep™ Mouse B Cell Enrichment Kit (Catalog #19754) for even faster cell isolations.